(P 97) TRANSCRIPTOMICS REVEALS THAT OVEREXPRESSION OF LNCRNA HOTTIP REGULATES PKC PROTEIN ACTIVATION OF THE RAP1 SIGNALING PATHWAY FOR SYNOVIAL SARCOMA CELL PROGRESSION

Objective: OBJECTIVE: The aim of this study was to elucidate whether LncRNA HOTTIP can affect synovial sarcoma progression and its mechanism, and to provide a basis for reducing synovial sarcoma metastasis and improving prognosis.

Methods: METHODS: SW982 cells were used as the target, and LncRNA HOTTIP was overexpressed to detect the proliferation, migration and invasion ability of SW982 cells, and then transcriptomic sequencing analysis was performed to find out the related pathway genes and proteins that affect tumor progression through LncRNA HOTTIP.

Results: RESULTS: The results of the study revealed that the proliferation, migration and invasion abilities of cells in the overexpression group were higher than those in the control group. Transcriptomics analysis showed that the up- and down-regulated differential genes were mainly involved in basic and tumor-related biological processes and pathways. For example, the up-regulated differential genes in GO analysis were mainly enriched in gene expression and cell proliferation and adhesion, and KEGG analysis showed that the up-regulated genes were mostly enriched in ribosomal, autophagy, ECM and other pathways, which suggests that overexpression of LncRNA HOTTIP promotes the migratory and invasive ability of SW982, and this could be due to the activation of a variety of tumor-associated pathways by the significant alteration of multiple genes expression levels. After that, we verified the transcriptomics results by qPCR and western blot, and the results were as expected.

Conclusion: CONCLUSION: The present study confirms that LncRNA HOTTIP may play a pro-carcinogenic role in the development and progression of synovial sarcoma.