(P 05) DEVELOPMENT OF SARCOMA ORGANOID MODELS WITH AIR-LIQUID INTERFACE ORGANOID CULTURES

Objective: Biological resources recapitulating the tumor microenvironment are essential for oncological research, but those of sarcoma are not sufficient. Organoid culture is a 3D culture method that enables the recapitulation of the tumor microenvironment and the success rate reported is higher than the 2D culture. The purpose of this study was to report our developing organoid models from human sarcomas using the air-liquid interface (ALI) organoid culture method and to introduce our efforts using the established organoids for the development of novel treatments for sarcoma.

Methods: The remaining various histological type of sarcoma specimens after surgical resection in our institute were cultured according to the ALI organoid culture method. After serial passages, we xenografted the organoids to NSG mice. We defined the establishment of organoids as cases where tumor formation was consistently observed after repeating this step more than twice. Using the developed tumors, we performed histological, genomic analyses and drug sensitivity analysis.

Results: Organoids from various sarcomas could be serially cultured and maintained including epithelioid sarcoma, dedifferentiated liposarcoma, synovial sarcoma, chondrosarcoma and so on. Developed orthotopic tumor xenografts were detected in the NSG mice in the in similar histological types. Thus, we established various sarcoma organoid lines with ALI organoid cultures. Their histological and genetic characteristics were sufficiently retained compared to the original tumors. Drug sensitivity tests are ongoing.

Conclusion: ALI organoid cultures is favorable culture method and development organoid models for various types of sarcoma. These sarcoma organoid models had the potential to become important tools for the development of new treatments.